Departmental Papers (Dental)

Document Type

Journal Article

Date of this Version

9-2012

Publication Source

Journal of Periodontology

Volume

83

Issue

9

Start Page

1139

Last Page

1148

DOI

10.1902/jop.2012.110506

Abstract

Background and objective

Surfaces and fluids can affect oral bacterial colonization. The aim of this study was to compare re-developing biofilms on natural teeth and dentures.

Methods

Supragingival plaque samples were taken from 55 dentate subjects and the denture teeth of 62 edentulous subjects before and after professional cleaning. Also, samples from 7 “teeth” in randomly selected quadrants were collected after 1, 2, 4 and 7 days of no oral hygiene. Samples were analyzed using checkerboard DNA-DNA hybridization. Counts and proportions of 41 bacterial taxa were determined at each time point and significant differences were sought using the Mann-Whitney test. Ecological succession was determined using a modified moving window analysis.

Results

Mean total DNA probe counts were similar pre-cleaning but were higher in dentate subjects at all post-cleaning visits (pStreptococcus mitis, Streptococcus oralisand Streptococcus mutans, whereas dentate subjects had higher proportions of Tannerella forsythia, Selenomonas noxia and Neisseria mucosa. By 2 days, mean counts of all taxa were higher in natural teeth and most remained higher at 7 days (pS. mitis and S. oralis by 1 day. N. mucosa, Veillonella parvula and Eikenella corrodens increased in both groups but later in edentate samples.

Conclusions

“Mature” natural and denture teeth biofilms have similar total numbers of bacteria but different species proportions. Post-cleaning biofilm re-development is more rapid and more complex on natural than denture teeth.

Copyright/Permission Statement

This is the pre-peer reviewed version of the following article: [Teles, F. R., Teles, R. P., Sachdeo, A., Uzel, N. G., Song, X. Q., Torresyap, G., . . . Socransky, S. S. (2012). Comparison of microbial changes in early redeveloping biofilms on natural teeth and dentures. Journal of Periodontology, 83(9), 1139-1148. doi:10.1902/jop.2012.110506], which has been published in final form at [http://dx.doi.org/10.1902/jop.2012.110506]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.

Comments

At the time of publication, author Flavia Teles was affiliated with the Harvard School of Dental Medicine. Currently, she is a faculty member at the School of Dental Medicine at the University of Pennsylvania.

Keywords

microbiota, biofilms, supragingival, dental plaque, tooth, dentures

Included in

Dentistry Commons

Share

COinS
 

Date Posted: 25 February 2022

This document has been peer reviewed.