Departmental Papers (Dental)
Microbial Shifts During Dental Biofilm Re-Development in the Absence of Oral Hygiene in Periodontal Health and Disease
Date of this Version
Journal of Clinical Periodontology
to monitor microbial shifts during dental biofilm re-development
Supra and subgingival plaque samples were taken separately from 28 teeth in 38 healthy and 17 periodontitis subjects at baseline and immediately after tooth cleaning. Samples were taken again from 7 teeth in randomly selected quadrants during 1, 2, 4 and 7 days of no oral hygiene. Samples were analyzed using checkerboard DNA-DNA hybridization. Species counts were averaged within subjects at each time point. Significant differences in counts between healthy and periodontitis subjects were sought using the Mann-Whitney test.
Total supra and subgingival counts were significantly higher in periodontitis on entry and reached or exceeded baseline values after day 2. Supragingival counts of Veillonella parvula, Fusobacterium nucleatum ss vincentii and Neisseria mucosa increased from 2 to 7 days. Subgingival counts were greater for Actinomyces, green and orange complex species. Significant differences between groups in supragingival counts occurred for 17 of 41 species at entry, 0 at day 7; for subgingival plaque these values were 39/41 taxa at entry, 17/41 at day 7.
Supragingival plaque re-development was similar in periodontitis and health, but subgingival species recolonization was more marked in periodontitis.
This is the pre-peer reviewed version of the following article: [Uzel, N. G., Teles, F. R., Teles, R. P., Song, X. Q., Torresyap, G., Socransky, S. S., & Haffajee, A. D. (2011). Microbial shifts during dental biofilm re‐development in the absence of oral hygiene in periodontal health and disease. Journal of Clinical Periodontology, 38(7), 612-620. doi:10.1111/j.1600-051X.2011.01730.x], which has been published in final form at [http://dx.doi.org/10.1111/j.1600-051X.2011.01730.x]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
oral bacteria, periodontal, health, periodontitis, biofilms, supragingival, subgingival
Uzel, N. G., Teles, F. R., Teles, R. P., Song, X. Q., Torresyap, G., Socransky, S. S., & Haffajee, A. D. (2011). Microbial Shifts During Dental Biofilm Re-Development in the Absence of Oral Hygiene in Periodontal Health and Disease. Journal of Clinical Periodontology, 38 (7), 612-620. http://dx.doi.org/10.1111/j.1600-051X.2011.01730.x
Date Posted: 25 February 2022
This document has been peer reviewed.
At the time of publication, author Flavia Teles was affiliated with the Forsyth Institute. Currently, she is a faculty member at the School of Dental Medicine at the University of Pennsylvania.