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We have developed a novel, all-electronic biosensor for opioids that consists of an engineered μ-opioid receptor protein, with high binding affinity for opioids, chemically bonded to a graphene field-effect transistor to read out ligand binding. A variant of the receptor protein that provided chemical recognition was computationally redesigned to enhance its solubility and stability in an aqueous environment. A shadow mask process was developed to fabricate arrays of hundreds of graphene transistors with average mobility of ∼1500 cm2 V–1 s–1 and yield exceeding 98%. The biosensor exhibits high sensitivity and selectivity for the target naltrexone, an opioid receptor antagonist, with a detection limit of 10 pg/mL.
This is an open access article published under an ACS AuthorChoice License, which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
Graphene, biosensor, field effect transistor, μ-opioid receptor, computational protein design
Lerner, M. B., Matsunaga, F., Han, G. H., Hong, S. J., Xi, J., Crook, A., Perez Aguilar, J., Park, Y. W., Saven, J. G., Liu, R., & Johnson, A. C. (2014). Scalable Production of Highly Sensitive Nanosensors Based on Graphene Functionalized With a Designed G Protein-Coupled Receptor. Nano Letters, 14 (5), 2709-2714. http://dx.doi.org/10.1021/nl5006349
Date Posted: 07 December 2016
This document has been peer reviewed.