Departmental Papers (CBE)

Document Type

Journal Article

Date of this Version



Pharmacological inhibition of dietary lipid absorption induces favorable changes in serum lipoprotein levels in patients that are at risk for cardiovascular disease and is considered an adjuvant or alternative treatment with HMG-CoA reductase inhibitors (statins). Here we demonstrate the feasibility of identifying novel inhibitors of intestinal lipid absorption using the zebrafish system. A pilot screen of an unbiased chemical library identified novel compounds that inhibited processing of fluorescent lipid analogues in live zebrafish larvae. Secondary assays identified those compounds suitable for testing in mammals and provided insight into mechanism of action, which for several compounds could be distinguished from ezetimibe, a drug used to inhibit cholesterol absorption in humans that broadly inhibited lipid absorption in zebrafish larvae. These findings support the utility of zebrafish screening assays to identify novel compounds that target complex physiological processes.


Suggested Citation:
Clifton, J.D., Lucumi, E., Myers, M.C., Napper, A., Hama, K. et al. (2010). "Identification of Novel Inhibitors of Dietary Lipid Absorption using Zebrafish." PLoS ONE Vol. 5(8). e12386.

doi: 10.1371/journal.pone.0012386



Date Posted: 04 November 2010

This document has been peer reviewed.