We retrospectively reviewed microbiology data from a tertiary care hospital in Botswana and found that Cryptococcus neoformans was cultured from 15% (193/1307) of all cerebrospinal (CSF) fluid specimens submitted for analysis, making it the most common diagnosed cause of meningitis in this population. Moreover, almost 70% of CSF samples with significant lymphocytosis did not yield a pathogen, suggesting that many causes of lymphocytic meningitis are going undiagnosed.