Document Type

Technical Report

Date of this Version

9-5-2006

Publication Source

PNAS (Proceedings of the National Academy of Sciences)

Volume

103

Issue

36

Start Page

13286

Last Page

13293

DOI

10.1073/pnas.0605528103

Abstract

Ethylene is a gaseous plant growth regulator that controls a multitude of developmental and stress responses. Recently, the levels of Arabidopsis EIN3 protein, a key transcription factor mediating ethylene-regulated gene expression, have been demonstrated to increase in response to the presence of ethylene gas. Furthermore, in the absence of ethylene, EIN3 is quickly degraded through a ubiquitin/proteasome pathway mediated by two F-box proteins, EBF1 and EBF2. Here we report the identification of ETHYLENE-INSENSITIVE5 as the 5′→3′ exoribonuclease XRN4. Specifically, we demonstrate that EIN5 is a component of the ethylene signal transduction cascade acting downstream of CTR1 that is required for ethylene-mediated gene expression changes. Furthermore, we find that the ethylene insensitivity of ein5 mutant plants is a consequence of the over-accumulation of EBF1 and EBF2 mRNAs resulting in the under-accumulation of EIN3 even in the presence of ethylene gas. Together, our results suggest that the role of EIN5 in ethylene perception is to antagonize the negative feedback regulation on EIN3 by promoting EBF1 and EBF2 mRNA decay, which consequently allows the accumulation of EIN3 protein to trigger the ethylene response.

Copyright/Permission Statement

© 2006 by The National Academy of Sciences of the USA.

This article has been published Open Access through the PNAS Open Access Option.

Comments

At the time of this publication, Dr. Gregory was affiliated with the Salk Institute, but he is now a faculty member at the University of Pennsylvania.

Keywords

Arabidposis, growth regulation, signal transduction

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Date Posted: 14 July 2017

This document has been peer reviewed.