Document Type

Technical Report

Date of this Version

3-30-2004

Publication Source

PNAS (Proceedings of the National Academy of Sciences)

Volume

101

Issue

13

Start Page

4554

Last Page

4559

DOI

10.1073/pnas.0400923101

Abstract

The structures of the bacterial RNA polymerase holoenzyme have provided detailed information about the intersubunit interactions within the holoenzyme. Functional analysis indicates that one of these is critical in enabling the holoenzyme to recognize the major class of bacterial promoters. It has been suggested that this interaction, involving the flap domain of the β subunit and conserved region 4 of the σ subunit, is a potential target for regulation. Here we provide genetic and biochemical evidence that the σ region 4/β-flap interaction is targeted by the transcription factor AsiA. Specifically, we show that AsiA competes directly with the β-flap for binding to σ region 4, thereby inhibiting transcription initiation by disrupting the σ region 4/β-flap interaction.

Copyright/Permission Statement

Copyright ©2004, The National Academy of Sciences.

Comments

At the time of this publication, Dr. Gregory was affiliated with Harvard Medical School, but he is now a faculty member of the University of Pennsylvania.

 

Date Posted: 14 July 2017

This document has been peer reviewed.