Effect of rapidly resorbable bone substitute materials on the temporal expression of the osteoblastic phenotype in vitro

Loading...
Thumbnail Image
Penn collection
Departmental Papers (BE)
Degree type
Discipline
Subject
bone substitute materials
calcium-alkali-orthophosphate ceramics
SaOS-2 cells
osteoblast differentiation
bioactive glass
Funder
Grant number
License
Copyright date
Distributor
Related resources
Author
Knabe, C.
Houshmand, A.
Berger, G.
Gildenhaar, R.
Kranz, I.
Stiller, M.
Contributor
Abstract

Ideally, bioactive ceramics for use in alveolar ridge augmentation should possess the ability to activate bone formation and, thus, cause the differentiation of osteoprogenitor cells into osteoblasts at their surfaces. Therefore, in order to evaluate the osteogenic potential of novel bone substitute materials, it is important to examine their effect on osteoblastic differentiation. This study examines the effect of rapidly resorbable calcium–alkali– orthophosphates on osteoblastic phenotype expression and compares this behavior to that of ß-tricalcium phosphate (TCP) and bioactive glass 45S5. Test materials were three materials (denominated GB14, GB9, GB9/25) with a crystalline phase Ca2KNa(PO4)2 and with a small amorphous portion containing either magnesium potassium phosphate (GB14) or silica phosphate (GB9 and GB9/25, which also contains Ca2P2O7); and a material with a novel crystalline phase Ca10K/Na7 (material denominated 352i). SaOS-2 human bone cells were grown on the substrata for 3, 7, 14, and 21 days, counted, and probed for an array of osteogenic markers. GB9 had the greatest stimulatory effect on osteoblastic proliferation and differentiation, suggesting that this material possesses the highest potency to enhance osteogenesis. GB14 and 352i supported osteoblast differentiation to the same or a higher degree than TCP, whereas, similar to bioactive glass 45S5, GB9/25 displayed a greater stimulatory effect on osteoblastic phenotype expression, indicating that GB9/25 is also an excellent material for promoting osteogenesis.

Advisor
Date Range for Data Collection (Start Date)
Date Range for Data Collection (End Date)
Digital Object Identifier
Series name and number
Publication date
2008-03-15
Journal title
Volume number
Issue number
Publisher
Publisher DOI
Journal Issue
Comments
Postprint version. Published in Journal of Biomedical Materials Research A, Volume 84, Issue 4, March 2008, pages 856-868. Publisher URL: http://dx.doi.org/10.1002/jbm.a.31383
Recommended citation
Collection