Remodeling of the plasma membrane and extracellular matrix (ECM) at discrete cellular locations plays important roles in various cellular processes including angiogenesis and cytokinesis. In the budding yeast Saccharomyces cerevisiae , membrane trafficking delivers enzymes essential for the synthesis of the cell-wall (yeast ECM) component chitin to the bud neck at different phases of the cell cycle. During early stages of budding, a Chs3-synthesized chitin ring is deposited at the base of the new bud that is required for bud-neck integrity and normal cell shape. During cytokinesis, actomyosin ring contraction is linked to the formation of a Chs2-synthesized chitinous disk to divide the mother and daughter cells called the primary septum. Chs3-synthesized chitin also plays an auxiliary rote to Chs2 during cytokinesis. Here, I show that the F-BAR protein Hof1 is involved in the endocytic removal of Chs3 from the bud neck alter chitin ring deposition and possibly later after cytokinesis. I also discuss work to show that Hof1 is involved in the localization and function of Inn1, a C2-domain containing protein essential for synthesis of the primary septum during cytokinesis.