Departmental Papers (BE)

Document Type

Conference Paper

Date of this Version

October 2002

Comments

Copyright 2002 IEEE. Reprinted from Proceedings of the Second Joint Engineering in Medicine and Biology Society/Biomedical Engineering Society Conference, (EMBS/BMES 2002), Volume 1, pages 674-675.
Publisher URL: http://ieeexplore.ieee.org/xpl/tocresult.jsp?isNumber=25190&page=22

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Abstract

Cardiovascular diseases are the nation's leading cause of death. Such diseases are caused by platelet response to collagen especially in the event of vascular injury leading to thrombosis. One of the platelet receptors known to bind to the collagen ligand is glycoprotein VI (GPVI) with co-receptor Fc receptor γ chain (FcRγ). By stably expressing the GPVI receptor in rat basophilic leukemia cells (RBL-2H3), which abundantly express FcRγ, but endogenously lack GPVI, studies have shown that GPVI-FcRγ is sufficient to confer adhesion as well as signaling responses to collagen as long as the receptor density is equivalent to that found on human platelets. While those investigations confirm that the GPVI receptor mediate binding to collagen under static conditions, they do not provide information on how the GPVI receptor interacts with collagen under dynamic conditions. In the present study we have used the GPVI-expressing RBL-2H3 cells to observe the kinetics of adhesion to collagen under hydrodynamic flow conditions in vitro using a parallel plate flow chamber coupled with video microscopy. We demonstrate that these cells do adhere to the surface at a low shear rate and do so at a greater adherent cell density than wild-type RBL-2H3 (WT-RBL) cells.

Keywords

GPVI-expressing RBL-2H3 cells, GPVI-173, collagen, cell adhesion, flow chamber

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Date Posted: 24 November 2004

This document has been peer reviewed.