Departmental Papers (BE)

Document Type

Journal Article

Date of this Version

2-6-2012

Publication Source

Circulation: Cardiovascular Imaging

Volume

5

Issue

2

Start Page

210

Last Page

217

DOI

10.1161/CIRCIMAGING.111.966317

Abstract

Background

We quantified absolute myocardial blood flow (MBF) using a spin-labeling MRI (SL-MRI) method after transplantation of endothelial cells (ECs) into the infarcted heart. Our aims were to study the temporal changes in MBF in response to EC transplantation and to compare regional MBF with contractile function (wall motion) and microvascular density.

Methods and Result

We first validated the SL-MRI method with the standard microsphere technique in normal rats. We then induced myocardial infarction in athymic rats and injected 5 million ECs (human umbilical vein endothelial cells) suspended in Matrigel or Matrigel alone (vehicle) along the border of the blanched infarcted area. At 2 weeks after myocardial infarction, MBF averaged over the entire slice (P=0.038) and in the infarcted region (P=0.0086) was significantly higher in EC versus vehicle group; the greater MBF was accompanied by an increase of microvasculature density in the infarcted region (P=0.0105 versus vehicle). At 4 weeks after myocardial infarction, MBF in the remote region was significantly elevated in EC-treated hearts (P=0.0277); this was accompanied by increased wall motion in this region assessed by circumferential strains (P=0.0075). Intraclass correlation coefficients and Bland-Altman plot revealed a good reproducibility of the SL-MRI method.

Conclusion

MBF in free-breathing rats measured by SL-MRI is validated by the standard color microsphere technique. SL-MRI allows quantification of temporal changes of regional MBF in response to EC treatment. The proof-of-principle study indicates that MBF is a unique and sensitive index to evaluate EC-mediated therapy for the infarcted heart.

Copyright/Permission Statement

Published in final edited form as: Circ Cardiovasc Imaging. 2012 March 1; 5(2): 210–217. doi:10.1161/CIRCIMAGING.111.966317.

Keywords

myocardial blood flow, spin-labeling, MRI, myocardial infarction, human umbilical vascular endothelial cells, microspheres, left ventricular ejection fraction

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Date Posted: 25 May 2016

This document has been peer reviewed.