Departmental Papers (BE)

Document Type

Journal Article

Date of this Version

6-2010

Publication Source

Pediatric Research

Volume

67

Issue

6

Start Page

585

Last Page

590

DOI

10.1203/PDR.0b013e3181dbc708

Abstract

Stretch is an essential mechanism for lung growth and development. Animal models in which fetal lungs have been chronically over or underdistended demonstrate a disrupted mix of type II and type I cells, with static overdistention typically promoting a type I cell phenotype. The Rho GTPase family, key regulators of cytoskeletal signaling, are known to mediate cellular differentiation in response to stretch in other organs. Using a well-described model of alveolar epithelial cell differentiation and a validated stretch device, we investigated the effects of supraphysiologic stretch on human fetal lung alveolar epithelial cell phenotype. Static stretch applied to epithelial cells suppressed type II cell markers (SP-B and Pepsinogen C, PGC), and induced type I cell markers (Caveolin-1, Claudin 7 and Plasminogen Activator Inhibitor-1, PAI-1) as predicted. Static stretch was also associated with Rho A activation. Furthermore, the Rho kinase inhibitor Y27632 decreased Rho A activation and blunted the stretch-induced changes in alveolar epithelial cell marker expression. Together these data provide further evidence that mechanical stimulation of the cytoskeleton and Rho activation are key upstream events in mechanotransduction-associated alveolar epithelial cell differentiation.

Copyright/Permission Statement

Published in final edited form as: Pediatric Research. 2010 June ; 67(6): 585–590. doi:10.1203/PDR.0b013e3181dbc708.

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Date Posted: 25 May 2016

This document has been peer reviewed.