Departmental Papers (BE)

Document Type

Journal Article

Date of this Version

2-23-2012

Abstract

We implement differential interference contrast (DIC) microscopy using high-speed synthetic aperture imaging that expands the passband of coherent imaging by a factor of 2.2. For an aperture synthesized coherent image, we apply for the numerical post-processing and obtain a high-contrast DIC image for arbitrary shearing direction and bias retardation. In addition, we obtain images at different depths without a scanning objective lens by numerically propagating the acquired coherent images. Our method achieves high-resolution and high-contrast 3-D DIC imaging of live biological cells. The proposed method will be useful for monitoring 3-D dynamics of intracellular particles.

Comments

Kim, M., Choi, Y., Fang-Yen, C., Sung, Y., Kim, K., Dasari, R. R., Feld, M. S., & Choi, W. (2012). Three-dimensional differential interference contrast microscopy using synthetic aperture imaging. Journal of Biomedical Optics, 17(2), 026003. doi: 10.1117/1.JBO.17.2.026003

Copyright 2012 Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.

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Date Posted: 28 February 2013

This document has been peer reviewed.