Date of this Version
We implement differential interference contrast (DIC) microscopy using high-speed synthetic aperture imaging that expands the passband of coherent imaging by a factor of 2.2. For an aperture synthesized coherent image, we apply for the numerical post-processing and obtain a high-contrast DIC image for arbitrary shearing direction and bias retardation. In addition, we obtain images at different depths without a scanning objective lens by numerically propagating the acquired coherent images. Our method achieves high-resolution and high-contrast 3-D DIC imaging of live biological cells. The proposed method will be useful for monitoring 3-D dynamics of intracellular particles.
Kim, M., Choi, Y., Fang-Yen, C., Sung, Y., Kim, K., Dasari, R. R., Feld, M. S., & Choi, W. (2012). Three-dimensional differential interference contrast microscopy using synthetic aperture imaging. Retrieved from http://repository.upenn.edu/be_papers/187
Date Posted: 28 February 2013
This document has been peer reviewed.