BBB Major Publications

Document Type

Journal Article

Date of this Version

5-2014

Publication Source

Langmuir

Volume

30

Issue

19

Start Page

5481

Last Page

5487

DOI

10.1021/la4047758

Abstract

Cell culture systems for studying the combined effects of matrix proteins and mechanical forces on the behavior of soft tissue cells have not been well developed. Here, we describe a new biomimetic cell culture system that allows for the study of mixtures of matrix proteins while controlling mechanical stiffness in a range that is physiological for soft tissues. This system consists of layer-by-layer (LbL)-assembled films of native matrix proteins atop mechanically tunable soft supports. We used hepatic stellate cells, which differentiate to myofibroblasts in liver fibrosis, for proof-of-concept studies. By culturing cells on collagen and lumican LbL-modified hydrogels, we demonstrate that this system is noncytotoxic and offers a valid control substrate, that the hydrogel determines the overall system mechanics, and that the addition of lumican to collagen influences the stellate cell phenotype. LbL-modified hydrogels offer the potential to study the influence of complex environmental factors on soft-tissue cells in culture.

Copyright/Permission Statement

ACS AuthorChoice - This is an open access article published under an ACS AuthorChoice License, which permits copying and redistribution of the article or any adaptations for non-commercial purposes.

Keywords

lumican, hepatic stellate cell, hydrogel, polyacrylamide, elastic modulus, liver fibrosis, layer-by-layer assembly

 

Date Posted:18 July 2017

This document has been peer reviewed.